OAC1 300586-90-7

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Molecular Weight: 237.26 OAC1 (Oct4-activating compound 1) can enhance the efficiency of reprogramming.


Biological Activity


OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4
and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1
enhances the pluripotent stem cells (iPSC) reprogramming efficiency and
accelerates the reprogramming process in the quartet reprogramming
factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic
fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the
quartet factors exhibits typical ESC morphology, gene-expression
pattern, and developmental potential. OAC1 seems to enhance
reprogramming efficiency via increasing transcription of the
Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA
demethylation. While it doesn`t inhibit the p53-p21 pathway or activate
the Wnt-β-catenin signaling. OAC1 may be used to enhance the
reprogramming of somatic cells to a pluripotent state.


Protocol(Only for Reference)


Cell Assay: [1]

Cell lines	Human ESCs transfected with human Oct4 and Nanog luciferase reporter genes
Concentrations	~1 μM
Incubation Time	24 h
Method	The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Baboon	Dog	Monkey	Rabbit	Guinea pig	Rat	Hamster	Mouse
Weight (kg)	12	10	3	1.8	0.4	0.15	0.08	0.02
Body Surface Area (m2)	0.6	0.5	0.24	0.15	0.05	0.025	0.02	0.007
Km factor	20	20	12	12	8	6	5	3

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×	mouse Km(3)	= 11.2 mg/kg
	rat Km(6)

Chemical Information

Molecular Weight (MW)	237.26
Formula	C14H11N3O
CAS No.	300586-90-7

Storage	3 years -20℃Powder
	6 months-80℃in solvent (DMSO, water, etc.)
Synonyms	BAS 00287861

Solubility (25°C) *	In vitro	DMSO	47 mg/mL (198.09 mM)
		Water	<1 mg/mL (
		Ethanol	21 mg/mL (88.51 mM)

Chemical Name	Benzamide, N-1H-pyrrolo[2,3-c]pyridin-5-yl-

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