Find Posaconazole Modulators, Calcitriol Modulators, Cyclosporin A Modulators on Industry Directory, Reliable Manufacturer/Supplier/Factory from China.

Inquiry Basket (0)
Home > Products > Others > Other > StemRegenin 1 (SR1) 1227633-49-9

StemRegenin 1 (SR1) 1227633-49-9

Product Description

.cp_wz table {border-top: 1px solid #ccc;border-left:1px solid #ccc; } .cp_wz table td{border-right: 1px solid #ccc; border-bottom: 1px solid #ccc; padding: 5px 0px 0px 5px;} .cp_wz table th {border-right: 1px solid #ccc;border-bottom: 1px solid #ccc; padding: 5px 0px 0px 5px;}


Molecular Weight: 429.54 StemRegenin 1 is an aryl hydrocarbon receptor (AhR) inhibitor with IC50 of 127 nM.


Biological Activity


StemRegenin 1 increases the number of CD34+ cells after 5 to 7 days with
an EC50 of 120 nM. Culture of mPB CD34+ cells with cytokines plus SR1
(1 μM) for 7 days increases the number of CD34+, CD133+, and CD90+
hematopoietic stem and progenitor cell populations 2.6-, 2.3-, and
10-fold, respectively compared to control cells. Continued culture with
SR1 (1 μM) for 3 weeks leads to an 11-fold increase in total nucleated
cells (TNC), a 73-fold increase in CD34+ cells as compared to control
cultures, and a 1118-fold increase in CD34+ cells relative to input
cells. Culture of 1×103 cord blood CD34+ cells for 5 weeks with SR1 (1 μM) results in the production of 1.69×106 colony forming cells.


SR1-induces CD34+ cell expansion acts by binding
and antagonizing AhR as evident by decreased CYP1B1 and AHRR mRNA
levels. SR1 (1 μM) treatment accelerates the proliferation of CD34+
cells and decreased the expression levels of VentX in human CD34+ cells.
Ectopic expression of VentX prevents SR1-induced expansion of CD34+
cells. Sequential coculture with bone morphogenetic protein 4 (20
ng/mL), PGE2 (2 μM), and SR1 (0.75 μM) lead to robust Macaca nemestrina
iPSC hematopoietic progenitor cell formation.
CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cells isolated on the basis of
CD34 expression and cultured in SR1 (0.75 μM) expands 3-fold and
maintained this long-term repopulating HSC phenotype.


SR1 promotes expansion of CB CD34+ cells that contribute to both early
and sustained engraftment NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice.


Protocol(Only for Reference)


Kinase Assay: [1]

AhR ligand binding assay Mouse livers are homogenized in buffer (25 mM MOPS, 2 mM EDTA, 0.02% NaN3, and 10% glycerol, pH 7.4) containing 20 mM sodium molybdate and protease Inhibitors and centrifuged at 100,000g for 1 h. ligand-treated lysates are incubated at room temperature for 20 min and then photolyzes at 8 cm with 402 nm UV light. Dextran-coated charcoal (1%) is added to the photolyzed samples, which are then centrifuged at 3000g for 10 min to remove free ligand. Labeled samples are resolved using 8% acrylamide-tricine-SDS-PAGE, transferred to PVDF membrane, and visualized using autoradiography. Labeled AHR bands are excised and counted using a γ counter.


Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)


Species Baboon Dog Monkey Rabbit Guinea pig Rat Hamster Mouse
Weight (kg) 12 10 3 1.8 0.4 0.15 0.08 0.02
Body Surface Area (m2) 0.6 0.5 0.24 0.15 0.05 0.025 0.02 0.007
Km factor 20 20 12 12 8 6 5 3

Animal A (mg/kg) = Animal B (mg/kg) multiplied by Animal B Km
Animal A Km



For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) × mouse Km(3) = 11.2 mg/kg
rat Km(6)



Chemical Information



Molecular Weight (MW) 429.54
Formula

C24H23N5OS

CAS No. 1227633-49-9

Storage 3 years -20℃Powder
6 months-80℃in solvent (DMSO, water, etc.)
Synonyms



Solubility (25°C) * In vitro DMSO 86 mg/mL (200.21 mM)
Water <1 mg/mL (
Ethanol <1 mg/mL (




Chemical Name Phenol, 4-[2-[[2-benzo[b]thien-3-yl-9-(1-methylethyl)-9H-purin-6-yl]amino]ethyl]-







Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.42954 4.2954 8.5908 12.8862


Contact us if you need more details on StemRegenin 1. We are ready to answer your questions on packaging, logistics, certification or any Other aspects about StemRegenin 1 1227633-49-9、1227633-49-9. If these products fail to match your need, please contact us and we would like to provide relevant information.

Product Categories : Others > Other

Email to this supplier
  • *Subject:
  • To:

  • *Messages:
    Your message must be between 20-8000 characters