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Home > Products > Others > Other > StemRegenin 1 (SR1) 1227633-49-9

StemRegenin 1 (SR1) 1227633-49-9

Product Description

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Molecular Weight: 429.54 StemRegenin 1 is an aryl hydrocarbon receptor (AhR) inhibitor with IC50 of 127 nM.

Biological Activity

StemRegenin 1 increases the number of CD34+ cells after 5 to 7 days with
an EC50 of 120 nM. Culture of mPB CD34+ cells with cytokines plus SR1
(1 μM) for 7 days increases the number of CD34+, CD133+, and CD90+
hematopoietic stem and progenitor cell populations 2.6-, 2.3-, and
10-fold, respectively compared to control cells. Continued culture with
SR1 (1 μM) for 3 weeks leads to an 11-fold increase in total nucleated
cells (TNC), a 73-fold increase in CD34+ cells as compared to control
cultures, and a 1118-fold increase in CD34+ cells relative to input
cells. Culture of 1×103 cord blood CD34+ cells for 5 weeks with SR1 (1 μM) results in the production of 1.69×106 colony forming cells.

SR1-induces CD34+ cell expansion acts by binding
and antagonizing AhR as evident by decreased CYP1B1 and AHRR mRNA
levels. SR1 (1 μM) treatment accelerates the proliferation of CD34+
cells and decreased the expression levels of VentX in human CD34+ cells.
Ectopic expression of VentX prevents SR1-induced expansion of CD34+
cells. Sequential coculture with bone morphogenetic protein 4 (20
ng/mL), PGE2 (2 μM), and SR1 (0.75 μM) lead to robust Macaca nemestrina
iPSC hematopoietic progenitor cell formation.
CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cells isolated on the basis of
CD34 expression and cultured in SR1 (0.75 μM) expands 3-fold and
maintained this long-term repopulating HSC phenotype.

SR1 promotes expansion of CB CD34+ cells that contribute to both early
and sustained engraftment NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice.

Protocol(Only for Reference)

Kinase Assay: [1]

AhR ligand binding assay Mouse livers are homogenized in buffer (25 mM MOPS, 2 mM EDTA, 0.02% NaN3, and 10% glycerol, pH 7.4) containing 20 mM sodium molybdate and protease Inhibitors and centrifuged at 100,000g for 1 h. ligand-treated lysates are incubated at room temperature for 20 min and then photolyzes at 8 cm with 402 nm UV light. Dextran-coated charcoal (1%) is added to the photolyzed samples, which are then centrifuged at 3000g for 10 min to remove free ligand. Labeled samples are resolved using 8% acrylamide-tricine-SDS-PAGE, transferred to PVDF membrane, and visualized using autoradiography. Labeled AHR bands are excised and counted using a γ counter.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species Baboon Dog Monkey Rabbit Guinea pig Rat Hamster Mouse
Weight (kg) 12 10 3 1.8 0.4 0.15 0.08 0.02
Body Surface Area (m2) 0.6 0.5 0.24 0.15 0.05 0.025 0.02 0.007
Km factor 20 20 12 12 8 6 5 3

Animal A (mg/kg) = Animal B (mg/kg) multiplied by Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) × mouse Km(3) = 11.2 mg/kg
rat Km(6)

Chemical Information

Molecular Weight (MW) 429.54


CAS No. 1227633-49-9

Storage 3 years -20℃Powder
6 months-80℃in solvent (DMSO, water, etc.)

Solubility (25°C) * In vitro DMSO 86 mg/mL (200.21 mM)
Water <1 mg/mL (
Ethanol <1 mg/mL (

Chemical Name Phenol, 4-[2-[[2-benzo[b]thien-3-yl-9-(1-methylethyl)-9H-purin-6-yl]amino]ethyl]-

Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.42954 4.2954 8.5908 12.8862

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